Then we used Clustal Omega and followed by Boxshade, we then converted the ALN file into the RTF format. The regions highlighted in black correspond to identical sequence reads. The sequence regions with amino acids of similar characteristics are highlighted in grey. Unconserved sequence regions correspond to the white areas.

 

Long segments highlighted in black indicate high conservation in all proteins, usually corresponding to specific catalytic domains. The Boxshade of eEF2K indicate high conservation in all species - in particular in the domains and motifs -with only small changes in the primary sequence giving specificity to each species. We also colour-coded all the known domains and motifs: calmodulin binding domain, alpha-kinase domain, the four SEL-1 repeats and the eEF2 interaction site.